Human FGF21(Fibroblast growth factor 21) ELISA Kit

Manual
  • Specifications
Product name

Human FGF21(Fibroblast growth factor 21) ELISA Kit

Catalogue No.

EH0130

Alias

FGF21(Fibroblast growth factor 21) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

FGF21 ELISA Kit allows for the in vitro quantitative determination of FGF21 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human FGF21(Fibroblast growth factor 21) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of FGF21 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF21 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-100 92
EDTA plasma(n=5) 86-104 93
heparin plasma(n=5) 94-103 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-88% 85-103% 85-104% 86-97%
EDTA plasma(n=5) 84-101% 87-98% 82-99% 82-93%
heparin plasma(n=5) 80-96% 83-98% 85-99% 82-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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