Human ETFDH(Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) ELISA Kit

Manual
  • Specifications
Product name

Human ETFDH(Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) ELISA Kit

Catalogue No.

EH0799

Alias

ETFDH(Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) ELISA Kit, ETF-QO ELISA Kit, ETF-ubiquinone oxidoreductase ELISA Kit, ETF dehydrogenase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ETFDH ELISA Kit allows for the in vitro quantitative determination of ETFDH concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78-5000pg/ml

Sensitivity

< 46.9pg/ml

Standard curve

Human ETFDH(Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ETFDH and the recovery rates were calculated by comparing the measured value to the expected amount of ETFDH in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-104 96
EDTA plasma(n=5) 86-104 97
heparin plasma(n=5) 87-96 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ETFDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-98% 92-103% 90-103% 85-104%
EDTA plasma(n=5) 86-96% 89-100% 83-101% 85-92%
heparin plasma(n=5) 93-98% 80-89% 87-100% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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