Human ELANE(Neutrophil elastase) ELISA Kit

Manual
  • Specifications
Product name

Human ELANE(Neutrophil elastase) ELISA Kit

Catalogue No.

EH0750

Alias

NE ELISA Kit, ELA2 ELISA Kit, ELANE ELISA Kit, Elastase-2 ELISA Kit, Leukocyte Elastase ELISA Kit, Medullasin ELISA Kit, Bone marrow serine protease ELISA Kit, ELA2granulocyte-derived elastase ELISA Kit, elastase 2, neutrophil ELISA Kit, elastase, neutrophil expressed ELISA Kit, Elastase-2 ELISA Kit, GE ELISA Kit, HLEelastase-2 ELISA Kit, HNE ELISA Kit, Human leukocyte elastase ELISA Kit, leukocyte elastase ELISA Kit, medullasin ELISA Kit, Medullasin ELISA Kit, NE ELISA Kit, neutrophil elastase ELISA Kit, PMN elastase ELISA Kit, PMN-E ELISA Kit, polymorphonuclear elastase ELISA Kit, SCN1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ELANE ELISA Kit allows for the in vitro quantitative determination of ELANE concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human ELANE(Neutrophil elastase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ELANE and the recovery rates were calculated by comparing the measured value to the expected amount of ELANE in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-104 96
EDTA plasma(n=5) 85-102 95
heparin plasma(n=5) 88-100 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ELANE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 85-102% 85-96% 85-102%
EDTA plasma(n=5) 82-99% 86-101% 87-98% 82-97%
heparin plasma(n=5) 83-99% 81-99% 83-99% 88-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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