Human CLDN2(Claudin-2) ELISA Kit

Manual
  • Specifications
Product name

Human CLDN2(Claudin-2) ELISA Kit

Catalogue No.

EH1341

Alias

CLDN2(Claudin-2) ELISA Kit, SP82 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CLDN2 ELISA Kit allows for the in vitro quantitative determination of CLDN2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.78-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human CLDN2(Claudin-2) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CLDN2 and the recovery rates were calculated by comparing the measured value to the expected amount of CLDN2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 93
EDTA plasma(n=5) 88-98 93
heparin plasma(n=5) 85-103 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CLDN2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 88-98% 85-103% 89-105%
EDTA plasma(n=5) 83-94% 82-101% 84-101% 82-95%
heparin plasma(n=5) 84-90% 83-92% 83-92% 81-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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