Human CHI3L1(Chitinase-3-like protein 1) ELISA Kit

Manual
  • Specifications
Product name

Human CHI3L1(Chitinase-3-like protein 1) ELISA Kit

Catalogue No.

EH0093

Alias

CHI3L1(Chitinase-3-like protein 1) ELISA Kit, GP39 ELISA Kit, Glycoprotein 39, Cartilage ELISA Kit, YKL-40 ELISA Kit, ASRT7 ELISA Kit, CGP-39 ELISA Kit, HC-gp39 ELISA Kit, YYL-40 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CHI3L1 ELISA Kit allows for the in vitro quantitative determination of CHI3L1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human CHI3L1(Chitinase-3-like protein 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CHI3L1 and the recovery rates were calculated by comparing the measured value to the expected amount of CHI3L1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-105 97
EDTA plasma(n=5) 92-102 97
heparin plasma(n=5) 85-103 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CHI3L1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 90-100% 85-102% 90-104%
EDTA plasma(n=5) 83-100% 83-95% 82-95% 82-101%
heparin plasma(n=5) 83-99% 81-98% 81-98% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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