Human CDKN2A(Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3) ELISA Kit

Manual
  • Specifications
Product name

Human CDKN2A(Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3) ELISA Kit

Catalogue No.

EH1050

Alias

CDKN2A(Cyclin Dependent Kinase Inhibitor 2A) ELISA Kit, p16INK4a ELISA Kit, ARF ELISA Kit, CDK4I ELISA Kit, INK4a ELISA Kit, MLM ELISA Kit, p14ARF ELISA Kit, p16 ELISA Kit, p19ARF ELISA Kit, ARF ELISA Kit, CDK4 inhibitor p16-INK4 ELISA Kit, CDKN2 ELISA Kit, cell cycle negative regulator beta ELISA Kit, CMM2P16-INK4A ELISA Kit, Cyclin-dependent kinase 4 inhibitor A ELISA Kit, cyclin-dependent kinase inhibitor 2A ELISA Kit, INK4 ELISA Kit, INK4A ELISA Kit, MLMP16INK4 ELISA Kit, MTS-1 ELISA Kit, MTS1P14 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CDKN2A ELISA Kit allows for the in vitro quantitative determination of CDKN2A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human CDKN2A(Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CDKN2A and the recovery rates were calculated by comparing the measured value to the expected amount of CDKN2A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 95-103 99
EDTA plasma(n=5) 85-103 95
heparin plasma(n=5) 87-103 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CDKN2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 93-103% 86-100% 85-98%
EDTA plasma(n=5) 91-97% 92-101% 84-99% 86-96%
heparin plasma(n=5) 83-96% 83-89% 82-95% 88-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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