Human APH1A(Gamma-secretase subunit APH-1A) ELISA Kit

Manual
  • Specifications
Product name

Human APH1A(Gamma-secretase subunit APH-1A) ELISA Kit

Catalogue No.

EH0887

Alias

APH1A(Gamma-secretase subunit APH-1A) ELISA Kit, APH-1 ELISA Kit, APH-1a ELISA Kit, aph-1alpha ELISA Kit, Aph-1alpha ELISA Kit, CGI-78 ELISA Kit, PSF ELISA Kit, anterior pharynx defective 1 homolog A ELISA Kit, Presenilin-stabilization factor ELISA Kit, Aph-1alpha ELISA Kit, Presenilin-stabilization factor ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

APH1A ELISA Kit allows for the in vitro quantitative determination of APH1A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

1.56-100ng/ml

Sensitivity

< 0.938ng/ml

Standard curve

Human APH1A(Gamma-secretase subunit APH-1A) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of APH1A and the recovery rates were calculated by comparing the measured value to the expected amount of APH1A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-98 93
EDTA plasma(n=5) 86-103 94
heparin plasma(n=5) 95-105 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APH1A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 85-102% 85-103% 87-104%
EDTA plasma(n=5) 84-98% 83-96% 84-99% 82-99%
heparin plasma(n=5) 81-99% 82-100% 83-94% 85-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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