Human ALDH4A1(Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial) ELISA Kit

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  • Specifications
Product name

Human ALDH4A1(Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial) ELISA Kit

Catalogue No.

EH1722

Alias

ALDH4A1 ELISA Kit, Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA Kit, P5C dehydrogenase ELISA Kit, Aldehyde dehydrogenase family 4 member A1 ELISA Kit, L-glutamate gamma-semialdehyde dehydrogenase ELISA Kit, ALDH4 ELISA Kit, P5CDH ELISA Kit, P5CD ELISA Kit, P5CDh ELISA Kit, P5CDhL ELISA Kit, P5CDhS ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ALDH4A1 ELISA Kit allows for the in vitro quantitative determination of ALDH4A1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human ALDH4A1(Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ALDH4A1 and the recovery rates were calculated by comparing the measured value to the expected amount of ALDH4A1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-103 94
EDTA plasma(n=5) 85-99 92
heparin plasma(n=5) 94-104 100
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALDH4A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 85-105% 89-100% 85-103%
EDTA plasma(n=5) 92-99% 88-100% 85-99% 83-100%
heparin plasma(n=5) 82-100% 86-92% 80-95% 84-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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