Human ALCAM(CD166 antigen) ELISA Kit

Manual
  • Specifications
Product name

Human ALCAM(CD166 antigen) ELISA Kit

Catalogue No.

EH0001

Alias

ALCAM ELISA Kit, CD166 ELISA Kit, MEMD ELISA Kit, activated leucocyte cell adhesion molecule ELISA Kit, CD166 antigen ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ALCAM ELISA Kit allows for the in vitro quantitative determination of ALCAM concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human ALCAM(CD166 antigen) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ALCAM and the recovery rates were calculated by comparing the measured value to the expected amount of ALCAM in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-105 92
EDTA plasma(n=5) 92-99 96
heparin plasma(n=5) 89-105 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 86-105% 86-103% 89-105%
EDTA plasma(n=5) 82-98% 85-101% 87-98% 82-97%
heparin plasma(n=5) 84-98% 80-94% 80-99% 82-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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