Human AKR1B10(Aldo-keto reductase family 1 member B10) ELISA Kit

Manual
  • Specifications
Product name

Human AKR1B10(Aldo-keto reductase family 1 member B10) ELISA Kit

Catalogue No.

EH1225

Alias

AKR1B10 ELISA Kit, Aldo-keto reductase family 1 member B10 ELISA Kit, Small intestine reductase ELISA Kit, SI reductase ELISA Kit, Aldose reductase-related protein ELISA Kit, ARP ELISA Kit, hARP ELISA Kit, ARL-1 ELISA Kit, Aldose reductase-like ELISA Kit

Detection method

Competitive ELISA, Coated with Antibody

Application

AKR1B10 ELISA Kit allows for the in vitro quantitative determination of AKR1B10 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.78-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human AKR1B10(Aldo-keto reductase family 1 member B10) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of AKR1B10 and the recovery rates were calculated by comparing the measured value to the expected amount of AKR1B10 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-99 93
EDTA plasma(n=5) 89-97 94
heparin plasma(n=5) 88-98 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKR1B10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-102% 91-103% 93-104% 90-101%
EDTA plasma(n=5) 82-98% 90-101% 82-100% 89-101%
heparin plasma(n=5) 85-99% 83-95% 84-96% 88-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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