Human ADAMTS1(A disintegrin and metalloproteinase with thrombospondin motifs 1) ELISA Kit

Manual
  • Specifications
Product name

Human ADAMTS1(A disintegrin and metalloproteinase with thrombospondin motifs 1) ELISA Kit

Catalogue No.

EH0031

Alias

ADAMTS1 ELISA Kit, METH1 ELISA Kit, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 1 ELISA Kit, ADAM-TS 1 ELISA Kit, ADAM-TS1 ELISA Kit, ADAMTS-1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ADAMTS1 ELISA Kit allows for the in vitro quantitative determination of ADAMTS1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human ADAMTS1(A disintegrin and metalloproteinase with thrombospondin motifs 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ADAMTS1 and the recovery rates were calculated by comparing the measured value to the expected amount of ADAMTS1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-105 100
EDTA plasma(n=5) 91-95 93
heparin plasma(n=5) 90-105 100
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADAMTS1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-105% 85-104% 85-105% 86-99%
EDTA plasma(n=5) 86-100% 89-98% 85-101% 88-101%
heparin plasma(n=5) 80-93% 84-100% 81-100% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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