Human ACTN3(Alpha-actinin-3) ELISA Kit

Manual
  • Specifications
Product name

Human ACTN3(Alpha-actinin-3) ELISA Kit

Catalogue No.

EH1251

Alias

ACTN3(Actinin Alpha 3) ELISA Kit, alpha-actinin skeletal muscle isoform 3 ELISA Kit, F-actin cross-linking protein ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ACTN3 ELISA Kit allows for the in vitro quantitative determination of ACTN3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human ACTN3(Alpha-actinin-3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ACTN3 and the recovery rates were calculated by comparing the measured value to the expected amount of ACTN3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-103 95
EDTA plasma(n=5) 89-105 98
heparin plasma(n=5) 85-105 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 91-104% 89-99% 86-102%
EDTA plasma(n=5) 85-99% 82-98% 87-99% 83-100%
heparin plasma(n=5) 82-92% 90-99% 93-100% 81-86%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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