Human ACTH(Corticotropin) ELISA Kit

Manual
  • Specifications
Product name

Human ACTH(Corticotropin) ELISA Kit

Catalogue No.

EH1067

Alias

ACTH(Adrenocorticotropic Hormone) ELISA Kit, Corticotropin ELISA Kit, Adrenocorticotropin ELISA Kit

Detection method

Competitive ELISA, Coated with Antigen

Application

ACTH ELISA Kit allows for the in vitro quantitative determination of ACTH concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human ACTH(Corticotropin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ACTH and the recovery rates were calculated by comparing the measured value to the expected amount of ACTH in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-102 95
EDTA plasma(n=5) 87-104 97
heparin plasma(n=5) 90-103 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 89-104% 86-102% 88-105%
EDTA plasma(n=5) 86-101% 83-101% 83-97% 85-100%
heparin plasma(n=5) 86-98% 82-98% 82-93% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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