Human ACA-IgM (Anti-Cardiolipin Antibody IgM) ELISA Kit

Manual
  • Specifications
Product name

Human ACA-IgM (Anti-Cardiolipin Antibody IgM) ELISA Kit

Catalogue No.

EH0479

Alias

ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit

Detection method

Sandwich ELISA, Double Antigen

Application

ACA-IgM ELISA Kit allows for the in vitro quantitative determination of ACA-IgM concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.375-24ug/ml

Sensitivity

< 0.225μg/ml

Standard curve

Human ACA-IgM (Anti-Cardiolipin Antibody IgM) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ACA-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgM in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-105 95
EDTA plasma(n=5) 87-102 96
heparin plasma(n=5) 95-101 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 86-104% 95-102% 85-96%
EDTA plasma(n=5) 83-99% 87-101% 87-95% 85-101%
heparin plasma(n=5) 82-99% 80-99% 85-97% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Hotline

027-87384275
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