Human ACA-IgA (Anti-Cardiolipin Antibody IgA) ELISA Kit

Manual
  • Specifications
Product name

Human ACA-IgA (Anti-Cardiolipin Antibody IgA) ELISA Kit

Catalogue No.

EH0477

Alias

ACA-IgA(Anti-Cardiolipin Antibody IgA) ELISA Kit

Detection method

Sandwich ELISA, Double Antigen

Application

ACA-IgA ELISA Kit allows for the in vitro quantitative determination of ACA-IgA concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

2.5-160ug/ml

Sensitivity

< 1.5μg/ml

Standard curve

Human ACA-IgA (Anti-Cardiolipin Antibody IgA) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ACA-IgA and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgA in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-104 99
EDTA plasma(n=5) 94-102 99
heparin plasma(n=5) 85-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-99% 86-97% 85-102% 93-105%
EDTA plasma(n=5) 83-92% 86-96% 82-100% 82-101%
heparin plasma(n=5) 82-96% 86-98% 84-97% 86-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Hotline

027-87384275
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