Bovine Osteocalcin ELISA Kit

Manual
  • Specifications
Product name

Bovine Osteocalcin ELISA Kit

Catalogue No.

EB0130

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Standard curve

Bovine Osteocalcin ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of and the recovery rates were calculated by comparing the measured value to the expected amount of in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 95-105 100
EDTA plasma(n=5) 92-101 95
heparin plasma(n=5) 86-100 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 87-105% 86-103% 88-101%
EDTA plasma(n=5) 85-96% 83-94% 88-99% 84-99%
heparin plasma(n=5) 80-100% 81-92% 89-97% 88-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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