HeLa cells were subjected to SDS PAGE followed by western blot with FNab09572(YTHDF1 antibody) at dilution of 1:500
IP Result of anti-YTHDF1 (IP:FNab09572, 4ug; Detection:FNab09572 1:1000) with mouse brain tissue lysate 2640ug.
Immunohistochemistry of paraffin-embedded human brain using FNab09572(YTHDF1 antibody) at dilution of 1:100
Background
Specifically recognizes and binds N6-methyladenosine(m6A)-containing mRNAs, and promotes mRNA translation efficiency(PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability(PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3(EIF3A or EIF3B) to facilitate translation initiation(PubMed:26046440).