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Vimentin antibody
- SPECIFICATIONS
- CITATIONS
- Product Name
- Vimentin antibody
- Catalogue No.
- FNab09409
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- vimentin
- Alternative Names
- FLJ36605 antibody, VIM antibody, Vimentin antibody
- UniProt ID
- P08670
- Observed MW
- 55 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
Hela cells were subjected to SDS PAGE followed by western blot with FNab09409(VIM antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary cancer using FNab09409(VIM antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells, using FNab09409(VIM antibody) at 1:25 dilution?
- Background
- Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
IF: 2.6
- Journal:
- Tissue and Cell
- Author:
- Department of Physiology, Faculty of Medicine, Karabuk University, Karabuk, Turkey
- Cited Date:
- 2023-08-04
- Product: