Products

Vimentin antibody

Synonyms:FLJ36605 antibody, VIM antibody, Vimentin antibody
Catalogue No.:FNab09409Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IF
Clonality:polyclonalIsotype:IgG
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Product Name
Vimentin antibody
Catalogue No.
FNab09409
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
vimentin
Alternative Names
FLJ36605 antibody, VIM antibody, Vimentin antibody
UniProt ID
P08670
Observed MW
55 kDa
Application
Tested Applications
ELISA, WB, IHC, IF
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
Hela cells were subjected to SDS PAGE followed by western blot with FNab09409(VIM antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary cancer using FNab09409(VIM antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells, using FNab09409(VIM antibody) at 1:25 dilution?
Background
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
IF: 2.6
Journal:
Tissue and Cell
Author:
Department of Physiology, Faculty of Medicine, Karabuk University, Karabuk, Turkey
Cited Date:
2023-08-04
Product: