Products
TSHR antibody
- Product Name
- TSHR antibody
- Catalogue No.
- FNab09045
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- thyroid stimulating hormone receptor
- Alternative Names
- CHNG1 antibody, hTSHR I antibody, LGR3 antibody, Thyrotropin receptor antibody, TSH R antibody, TSHR antibody
- UniProt ID
- P16473
- Observed MW
- 62-100 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:20- 1:50
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09045(TSHR antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat brain using FNab09045(TSHR antibody) at dilution of 1:100
- Background
- The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody can detect glycosylated subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)
