HeLa cells were subjected to SDS PAGE followed by western blot with FNab08367(SUB1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human pancreas cancer using FNab08367(SUB1 antibody) at dilution of 1:50
Immunofluorescence analysis of A549 cells using FNab08367(SUB1 antibody).
Background
SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. This antibody is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).