Jurkat cells were subjected to SDS PAGE followed by western blot with FNab07994( SMAD3 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab07994( SMAD3 Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using FNab07994 ( SMAD3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.