Products
RAF1 antibody
- Product Name
- RAF1 antibody
- Catalogue No.
- FNab07087
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- v-raf-1 murine leukemia viral oncogene homolog 1
- Alternative Names
- c-Raf antibody, CMD1NN antibody, CRAF antibody, NS5 antibody, Raf-1 antibody
- UniProt ID
- P04049
- Observed MW
- 75 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab07087(RAF1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat heart using FNab07087(RAF1 antibody) at dilution of 1:50
- Background
- This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
