Products

RAD18 antibody

Synonyms:RNF73 antibody
Catalogue No.:FNab07075Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
RAD18 antibody
Catalogue No.
FNab07075
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
RAD18 homolog
Alternative Names
RNF73 antibody
UniProt ID
Q9NS91
Observed MW
80 kDa
Application
Tested Applications
ELISA, WB, IHC, IF
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07075( RAD18 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung using FNab07075(RAD18 antibody) at dilution of 1:50
Immunofluorescence analysis of U2OS cells using FNab07075( RAD18 Antibody) at dilution of 1:100
Background
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.