Jurkat cells were subjected to SDS PAGE followed by western blot with FNab06461(PIP4K2A antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat kidney using FNab06461(PIP4K2A antibody) at dilution of 1:50
Immunofluorescence analysis of A549 cells using PIP4K2A antibody (FNab06461)
Background
Phosphatidylinositol-5, 4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5, 4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.