Products
PHF2 antibody
- Product Name
- PHF2 antibody
- Catalogue No.
- FNab06384
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- PHD finger protein 2
- Alternative Names
- GRC5 antibody, JHDM1E antibody, KIAA0662 antibody, PHD finger protein 2 antibody, PHF2 antibody
- UniProt ID
- O75151
- Observed MW
- 100 kDa, 150 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:200-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab06384(PHF2 Antibody) at dilution of 1:500
IP Result of anti-PHF2 (IP:FNab06384, 4ug; Detection:FNab06384 1:500) with HeLa cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using FNab06384(PHF2 Antibody) at dilution of 1:50
- Background
- Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3(H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3(H3K4me3) at rDNA promoters and promotes expression of rDNA.
