anti- p65/RELA antibody

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Product Information
Product name

anti- p65/RELA antibody

Catalogue No.

FNab09875

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 24 months(Avoid repeated freeze / thaw cycles.)

Background

Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Immunogen Information
Immunogen

v-rel reticuloendotheliosis viral oncogene homolog A(avian)

Synonyms

NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65

Calculated MW

65 kDa

Application
Specificity

Human, Rat

Tested Application

ELISA, IHC, IF, WB

Recommended dilution

WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200

Uniprot information
Uniprot ID

Q04206

Images

anti- p65/RELA antibody 1

Immunohistochemistry of paraffin-embedded rat lung using FNab09875(p65 antibody) at dilution of 1:50


anti- p65/RELA antibody 1

HeLa cells were subjected to SDS PAGE followed by western blot with FNab09875(p65 antibody) at dilution of 1:500

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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Hotline

027-87384275
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