Products
MRPL28 antibody
- Product Name
- MRPL28 antibody
- Catalogue No.
- FNab05325
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- mitochondrial ribosomal protein L28
- Alternative Names
- MAAT1 antibody
- UniProt ID
- Q13084
- Observed MW
- 30 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100; IP: 1:50 - 1:200
Validated Images
A375 cells were subjected to SDS PAGE followed by western blot with FNab05325(MRPL28 antibody) at dilution of 1:1000
IP Result of anti-MRPL28 (IP:FNab05325, 4ug; Detection:FNab05325 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human gastric cancer using FNab05325(MRPL28 antibody) at dilution of 1:50
- Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.
