Products
MPRIP antibody
- Product Name
- MPRIP antibody
- Catalogue No.
- FNab05296
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- myosin phosphatase Rho interacting protein
- Alternative Names
- KIAA0864 antibody, MRIP antibody, RHOIP3 antibody
- UniProt ID
- Q6WCQ1
- Observed MW
- 116-140 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with FNab05296(MPRIP antibody) at dilution of 1:800
IP Result of anti-MPRIP (IP:FNab05296, 4ug; Detection:FNab05296 1:500) with SKOV-3 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human ovary using FNab05296(MPRIP antibody) at dilution of 1:100
Immunofluorescent analysis of Hela cells, using FNab05296(MPRIP antibody) at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Background
- Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
