Products
LMP7 antibody
- Product Name
- LMP7 antibody
- Catalogue No.
- FNab04811
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
- Alternative Names
- beta5i antibody, D6S216 antibody, D6S216E antibody, LMP7 antibody, Low molecular mass protein 7 antibody, Macropain subunit C13 antibody, Proteasome component C13 antibody, Proteasome subunit beta 5i antibody, Proteasome subunit beta type 8 antibody, PSMB5i antibody, PSMB8 antibody, RING10 antibody, Y2 antibody
- UniProt ID
- P28062
- Observed MW
- 24 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
mouse kidney tissue were subjected to SDS PAGE followed by western blot with FNab04811(PSMB8 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lymphoma using FNab04811(PSMB8 antibody) at dilution of 1:50
Immunofluorescence analysis of A549 cells using FNab04811(PSMB8 antibody). Blue: DAPI for nuclear staining.
- Background
- The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
