anti- LCK antibody

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Product Information
Product name

anti- LCK antibody

Alternative name

LCK Antibody

Catalogue No.

FNab04727

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

Monoclonal

Isotype

IgG2a

Description

LCK antibody: Mouse Monoclonal antibody to LCK

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Background

Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs(ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.

Immunogen Information
Immunogen

lymphocyte-specific protein tyrosine kinase

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

Calculated MW

50-60 kDa

Application
Specificity

Human, rat

Tested Application

ELISA, WB

Recommended dilution

WB: 1:500-1:3000

Uniprot information
Gene Name

LCK

Uniprot ID

P06239

Images

anti- LCK antibody 1

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab04727 (LCK antibody) at dilution of 1:1000

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Hotline

027-87384275
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