Products
Histone H2A.X antibody
- Product Name
- Histone H2A.X antibody
- Catalogue No.
- FNab03885
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- H2A histone family, member X
- Alternative Names
- H2A histone family antibody, member X antibody, H2A.X antibody, H2A/X antibody, H2AFX antibody, H2AX antibody, Histone H2A.x antibody
- UniProt ID
- P16104
- Observed MW
- 16 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:100 - 1:200; IF: 1:50 - 1:200
Validated Images
HL-60 cells were subjected to SDS PAGE followed by western blot with FNab03885(H2AFX antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer using FNab03885(H2AFX antibody) at dilution of 1:100
- Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
