Products

GPX4 antibody

Catalogue No.:FNab03622Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IF
Clonality:polyclonalIsotype:IgG
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Product Name
GPX4 antibody
Catalogue No.
FNab03622
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
glutathione peroxidase 4 (phospholipid hydroperoxidase)
UniProt ID
P36969
Observed MW
25 kDa
Application
Tested Applications
ELISA, WB, IHC, IF
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
mouse kidney tissue were subjected to SDS PAGE followed by western blot with FNab03622(GPX4 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver injury using FNab03622(GPX4 antibody) at dilution of 1:50
Background
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.
IF: 3.9
Journal:
BMC Complementary Medicine and Therapies
Author:
Department of Ophthalmology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111DaDe Road, Guangzhou, Guangdong, 510120, China
Cited Date:
2023-12-08
Product:
IF: 3.2
Journal:
Toxicology in Vitro
Author:
Ankara University, Faculty of Pharmacy, Department of Biochemistry, 06560 Anadolu, Ankara, Turkey
Cited Date:
2023-11-17
Product: