Products

GNAI1 antibody

Catalogue No.:FNab03531Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, FC, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
GNAI1 antibody
Catalogue No.
FNab03531
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
guanine nucleotide binding protein(G protein), alpha inhibiting activity polypeptide 1
UniProt ID
P63096
Observed MW
40 kDa
Application
Tested Applications
ELISA, WB, IHC, FC, IF
Recommended dilution
WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
human brain tissue were subjected to SDS PAGE followed by western blot with FNab03531(GNAI1 antibody) at dilution of 1:1600
Immunohistochemistry of paraffin-embedded human gliomas using FNab03531(GNAI1 antibody) at dilution of 1:100
Background
Guanine nucleotide-binding proteins(G proteins) function as transducers downstream of G protein-coupled receptors(GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins(PubMed:8774883, PubMed:18434541). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels(By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis(PubMed:17635935).