Products

E2F4 antibody

Catalogue No.:FNab02602Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
E2F4 antibody
Catalogue No.
FNab02602
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
E2F transcription factor 4, p107/p130-binding
UniProt ID
Q16254
Observed MW
60-70 kDa
Application
Tested Applications
ELISA, WB, IHC, IP
Recommended dilution
WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
A431 cells were subjected to SDS PAGE followed by western blot with FNab02602(E2F4 antibody) at dilution of 1:1000
IP Result of anti-E2F4 (IP:FNab02602, 3ug; Detection:FNab02602 1:1000) with mouse brain tissue lysate 3600ug.
Immunohistochemistry of paraffin-embedded human lymphoma using FNab02602(E2F4 antibody) at dilution of 1:100
Background
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.