Products

CTCF antibody

Catalogue No.:FNab02049Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
CTCF antibody
Catalogue No.
FNab02049
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
CCCTC-binding factor (zinc finger protein)
UniProt ID
P49711
Observed MW
135 kDa
Application
Tested Applications
ELISA, WB, IHC, IP
Recommended dilution
WB: 1:1000 - 1:2000; IHC: 1:50 - 1:200; ChIP : 1:50 - 1:200
Validated Images
human brain tissue were subjected to SDS PAGE followed by western blot with FNab02049(CTCF antibody) at dilution of 1:1000
IP Result of anti-CTCF (IP:FNab02049, 5ug; Detection:FNab02049 1:300) with MCF-7 cells lysate 2560ug.
Immunohistochemistry of paraffin-embedded mouse brain using FNab02049(CTCF antibody) at dilution of 1:100
Background
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.