human colon tissue were subjected to SDS PAGE followed by western blot with FNab00907(BLMH antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using FNab00907(BLMH antibody) at dilution of 1:50
Immunofluorescence analysis of HeLa cells using FNab00907(BLMH antibody) at dilution of 1:50
Background
Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.