BLMH antibody

Catalogue No.:FNab00907Reactivity:Human, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
BLMH antibody
Catalogue No.
FNab00907
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
bleomycin hydrolase
UniProt ID
Q13867
Observed MW
50 kDa
Application
Tested Applications
ELISA, WB, IHC, IF
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:20 - 1:50
Validated Images
human colon tissue were subjected to SDS PAGE followed by western blot with FNab00907(BLMH antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using FNab00907(BLMH antibody) at dilution of 1:50
Immunofluorescence analysis of HeLa cells using FNab00907(BLMH antibody) at dilution of 1:50
Background
Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.