ATP5H antibody

Synonyms:ATP5H antibody, ATP5JD antibody, ATPase subunit d antibody, ATPQ antibody
Catalogue No.:FNab10033Reactivity:Human, Mouse
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
ATP5H antibody
Catalogue No.
FNab10033
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Alternative Names
ATP5H antibody, ATP5JD antibody, ATPase subunit d antibody, ATPQ antibody
UniProt ID
O75947
Observed MW
19-22 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500-1:2000; IHC: 1:50-1:500
Validated Images
A549 cells were subjected to SDS PAGE followed by western blot with FNab10033(ATP5H antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney using FNab10033(ATP5H antibody) at dilution of 1:100
Background
Mitochondrial membrane ATP synthase(F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1)-containing the extramembraneous catalytic core, and F(0)-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.