Products
AlaRS antibody
- Product Name
- AlaRS antibody
- Catalogue No.
- FNab00276
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- alanyl-tRNA synthetase
- Alternative Names
- AARS antibody, Alanine tRNA ligase antibody, alanyl tRNA synthetase antibody, AlaRS antibody
- UniProt ID
- P49588
- Observed MW
- 107 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200; IP: 1:200-1:2000
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00276(AARS antibody) at dilution of 1:400
IP Result of anti-AlaRS (IP:FNab00276, 4ug; Detection:FNab00276 1:500) with HepG2 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human liver using FNab00276(AARS antibody) at dilution of 1:100
- Background
- AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).
