Products

AIF antibody

Synonyms:AIF antibody, AIFM1 antibody, PDCD8 antibody
Catalogue No.:FNab00235Reativity:Human, Mouse
Host:RabbitTested Application:ELISA, IHC, WB, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
AIF antibody
Catalogue No.
FNab00235
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
apoptosis-inducing factor, mitochondrion-associated, 1
Alternative Names
AIF antibody, AIFM1 antibody, PDCD8 antibody
UniProt ID
O95831
Observed MW
67 kDa
Application
Tested Applications
ELISA, IHC, WB, IF
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab00235(AIF antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human esophagus tissue slide using FNab00235( AIF Antibody) at dilution of 1:100
Immunofluorescence analysis of U2OS cells using FNab00235( AIF Antibody). Blue: DAPI for nuclear staining.
Background
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.