Products

AFF1 antibody

Synonyms:AFF1 antibody, MLLT2 antibody, AF4 MLL antibody, PBM1 antibody, AF 4 antibody
Catalogue No.:FNab00195Reativity:Human, rat
Host:RabbitTested Application:ELISA, WB, IHC, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
AFF1 antibody
Catalogue No.
FNab00195
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
AF4/FMR2 family, member 1
Alternative Names
AFF1 antibody, MLLT2 antibody, AF4 MLL antibody, PBM1 antibody, AF 4 antibody
UniProt ID
P51825
Observed MW
131 kDa
Application
Tested Applications
ELISA, WB, IHC, IF
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100
Validated Images
K-562 cells were subjected to SDS PAGE followed by western blot with FNab00195(AFF1-Specific antibody) at dilution of 1:1000
Immunofluorescence analysis of U2OS cells using FNab00195(AFF1-Specific antibody).
Background
This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.