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Aconitase 1 antibody
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Synonyms: | ACO1 antibody, Aconitase antibody, Aconitase 1 antibody, aconitase 1 antibody, soluble antibody, ACONS antibody, Citrate hydro lyase antibody, Ferritin repressor protein antibody, IRE BP 1 antibody, IREB1 antibody, IREBP antibody, IREBP1 antibody, Iron regulatory protein 1 antibody, IRP1 antibody | ||
Catalogue No.: | FNab00087 | Reativity: | Human, Mouse, Rat |
Host: | Rabbit | Tested Application: | ELISA, WB, IHC |
Clonality: | polyclonal | Isotype: | IgG |
- SPECIFICATIONS
- Product Name
- Aconitase 1 antibody
- Catalogue No.
- FNab00087
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- aconitase 1, soluble
- Alternative Names
- ACO1 antibody, Aconitase antibody, Aconitase 1 antibody, aconitase 1 antibody, soluble antibody, ACONS antibody, Citrate hydro lyase antibody, Ferritin repressor protein antibody, IRE BP 1 antibody, IREB1 antibody, IREBP antibody, IREBP1 antibody, Iron regulatory protein 1 antibody, IRP1 antibody
- UniProt ID
- P21399
- Observed MW
- 120 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Validated Images
Hela cells were subjected to SDS PAGE followed by western blot with FNab00087(ACO1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using FNab00087(ACO1 antibody) at dilution of 1:100
- Background
- The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants