Products

4EBP1 antibody

Synonyms:4E BP1 antibody, 4EBP1 antibody, BP 1 antibody, eIF4E binding protein 1 antibody, EIF4EBP1 antibody, PHAS I antibody
Catalogue No.:FNab00006Reativity:Human
Host:MouseTested Application:ELISA, WB, IF
Clonality:monoclonalIsotype:IgG2b
  • SPECIFICATIONS
Product Name
4EBP1 antibody
Catalogue No.
FNab00006
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG2b
Clone ID
6C4
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
eukaryotic translation initiation factor 4E binding protein 1
Alternative Names
4E BP1 antibody, 4EBP1 antibody, BP 1 antibody, eIF4E binding protein 1 antibody, EIF4EBP1 antibody, PHAS I antibody
UniProt ID
Q13541
Observed MW
12 kDa
Application
Tested Applications
ELISA, WB, IF
Recommended dilution
WB: 1:500-1:2000; IF: 1:20-1:200
Validated Images
K-562 cells were subjected to SDS PAGE followed by western blot with FNab00006(4EBP1 antibody) at dilution of 1:2000
Immunofluorescent analysis of Hela cells using FNab00006 ( 4EBP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
Background
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.