Products
14-3-3 antibody
- Product Name
- 14-3-3 antibody
- Catalogue No.
- FNab00001
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
- Alternative Names
- 14 3 3 antibody, 14 3 3 protein T cell antibody, 14 3 3 protein tau antibody, 14 3 3 protein theta antibody, 1C5 antibody, HS1 antibody, Protein HS1 antibody, YWHAQ antibody
- UniProt ID
- P27348
- Observed MW
- 28 kDa
Application
- Tested Applications
- ELISA, IF, IP, IHC, WB
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
Hela cell were subjected to SDS PAGE followed by western blot with FNab00001(14-3-3 Antibody) at dilution of 1:1000
IP Result of anti 14-3-3(IP:FNab00001, 5μg; Detection:FNab00001 1:500)with mouse lung tissue lysate 4000μg.
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using FNab00001(14-3-3 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using FNab00001(14-3-3 Antibody) at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Background
- 14-3-3 theta(also known as YWHAQ, or 14-3-3 tau) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms(gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo-and hetero-dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 theta.
