HeLa cells were subjected to SDS PAGE followed by western blot with FNab00375(PRKAA1 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab00375(AMPK alpha Antibody) at dilution of 1:200
Immunofluorescent analysis of HepG2 cells using FNab00375( AMPK alpha Antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Background
The mammalian 5-prime-AMP-activated protein kinase(AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase(SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms produced by alternative splicing.