Human VIP(VIP peptides) ELISA Kit

说明书
  • 规格
Product name

Human VIP(VIP peptides) ELISA Kit

Catalogue No.

EH0694

Alias

VIP(vasoactive intestinal peptide) ELISA Kit, VIP peptides ELISA Kit, PHM27 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

VIP ELISA Kit allows for the in vitro quantitative determination of VIP concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.813-500pg/ml

Sensitivity

< 4.688pg/ml

Standard curve

Human VIP(VIP peptides) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of VIP and the recovery rates were calculated by comparing the measured value to the expected amount of VIP in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-100 92
EDTA plasma(n=5) 86-102 94
heparin plasma(n=5) 87-102 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 90-100% 90-105% 92-104%
EDTA plasma(n=5) 82-99% 92-101% 82-88% 87-98%
heparin plasma(n=5) 80-86% 80-99% 84-99% 82-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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