Human VIM(Vimentin) ELISA Kit

说明书
  • 规格
Product name

Human VIM(Vimentin) ELISA Kit

Catalogue No.

EH1166

Alias

VIM(Vimentin) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

VIM ELISA Kit allows for the in vitro quantitative determination of VIM concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.813-500ng/ml

Sensitivity

< 4.688ng/ml

Standard curve

Human VIM(Vimentin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of VIM and the recovery rates were calculated by comparing the measured value to the expected amount of VIM in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-102 92
EDTA plasma(n=5) 92-102 97
heparin plasma(n=5) 87-102 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VIM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-100% 87-102% 85-105% 86-104%
EDTA plasma(n=5) 84-100% 84-97% 85-95% 82-97%
heparin plasma(n=5) 89-99% 83-95% 83-98% 81-88%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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