Human TIMP-4(Metalloproteinase inhibitor 4) ELISA Kit

说明书
  • 规格
Product name

Human TIMP-4(Metalloproteinase inhibitor 4) ELISA Kit

Catalogue No.

EH0297

Alias

TIMP-4 ELISA Kit, TIMP metallopeptidase inhibitor 4 ELISA Kit, tissue inhibitor of metalloproteinase 4 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TIMP-4 ELISA Kit allows for the in vitro quantitative determination of TIMP-4 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78-5000pg/ml

Sensitivity

< 46.875pg/ml

Standard curve

Human TIMP-4(Metalloproteinase inhibitor 4) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TIMP-4 and the recovery rates were calculated by comparing the measured value to the expected amount of TIMP-4 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 96
EDTA plasma(n=5) 85-102 94
heparin plasma(n=5) 85-104 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIMP-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 96-105% 86-97% 94-103%
EDTA plasma(n=5) 85-101% 84-101% 92-100% 82-98%
heparin plasma(n=5) 81-99% 88-100% 82-97% 83-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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