Human TIMP-3(Metalloproteinase inhibitor 3) ELISA Kit

说明书
  • 规格
Product name

Human TIMP-3(Metalloproteinase inhibitor 3) ELISA Kit

Catalogue No.

EH0296

Alias

TIMP-3 ELISA Kit, MIG-5 protein ELISA Kit, Protein MIG-5 ELISA Kit, SFD ELISA Kit, TIMP metallopeptidase inhibitor 3 ELISA Kit, tissue inhibitor of metalloproteinase 3 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TIMP-3 ELISA Kit allows for the in vitro quantitative determination of TIMP-3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human TIMP-3(Metalloproteinase inhibitor 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TIMP-3 and the recovery rates were calculated by comparing the measured value to the expected amount of TIMP-3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-104 94
EDTA plasma(n=5) 87-102 96
heparin plasma(n=5) 86-102 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIMP-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 86-105% 92-102% 87-101%
EDTA plasma(n=5) 84-92% 82-100% 85-99% 83-100%
heparin plasma(n=5) 82-100% 83-92% 81-99% 83-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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