Human TGFα(Transforming Growth Factor α) ELISA Kit

说明书
  • 规格
Product name

Human TGFα(Transforming Growth Factor α) ELISA Kit

Catalogue No.

EH0284

Alias

TGF-α(Transforming Growth Factor α) ELISA Kit, TGFα ELISA Kit, TGFA ELISA Kit, protransforming growth factor alpha ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TGFα ELISA Kit allows for the in vitro quantitative determination of TGFα concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human TGFα(Transforming Growth Factor α) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TGFα and the recovery rates were calculated by comparing the measured value to the expected amount of TGFα in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-105 96
EDTA plasma(n=5) 88-102 93
heparin plasma(n=5) 85-103 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-92% 86-97% 85-99% 89-103%
EDTA plasma(n=5) 88-93% 90-100% 83-101% 90-99%
heparin plasma(n=5) 85-96% 80-100% 83-98% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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