Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) ELISA Kit

说明书
  • 规格
Product name

Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) ELISA Kit

Catalogue No.

EH0991

Alias

Smad1 ELISA Kit, MADH1(Mothers Against Decapentaplegic Homolog 1) ELISA Kit, BSP1 ELISA Kit, BSP-1 ELISA Kit, hSMAD1 ELISA Kit, JV4-1SMAD, mothers against DPP homolog 1(Drosophila) ELISA Kit, MAD homolog 1 ELISA Kit, Mothers against DPP homolog 1 ELISA Kit, SMAD family member 1SMAD 1 ELISA Kit, SMAD, mothers against DPP homolog 1 ELISA Kit, Smad1 ELISA Kit, TGF-beta signaling protein 1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

Smad1/MADH1 ELISA Kit allows for the in vitro quantitative determination of Smad1/MADH1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Smad1/MADH1 and the recovery rates were calculated by comparing the measured value to the expected amount of Smad1/MADH1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-104 98
EDTA plasma(n=5) 85-98 92
heparin plasma(n=5) 92-103 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Smad1/MADH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 87-105% 85-104% 86-100%
EDTA plasma(n=5) 84-99% 84-101% 88-98% 82-101%
heparin plasma(n=5) 80-93% 80-100% 85-100% 83-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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